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En protokol til brug af gen sæt berigelse analyse til at identificere den passende dyremodel for Translationel forskning
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Journal JoVE Biologie
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

En protokol til brug af gen sæt berigelse analyse til at identificere den passende dyremodel for Translationel forskning

DOI:
10.3791/55768-v

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09:35 min

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August 16, 2017

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, , , , ,
1Department of Experimental Toxicology and ZEBET,German Federal Institute for Risk Assessment (BfR), 2Department of Clinical Pharmacology and Toxicology,Charité-Universitätsmedizin Berlin

Chapitres

  • 00:05Titre
  • 00:54Performing the Game Set Enrichment Analysis (GSEA)
  • 05:46Comparing the GSEA Results and Identifying the Optimal Animal Model
  • 07:55Results: Overlap of Pathway Regulation Identifies Suitable and Unsuitable Animal Models
  • 08:49Conclusion

Summary

Traduction automatique

Traduction automatique

Vi leverer en standardiseret protokol for brug af gen sæt berigelse analyse af transkriptom data til at identificere en ideel musemodel for Translationel forskning.
Denne protokol kan bruges med DNA microarray og RNA sequencing data og kan yderligere udvides til at omfatte andre omik data, hvis der foreligger data.

Tags

Keywords: Gene Set Enrichment AnalysisAnimal ModelTranslational ResearchGenome DataMouse ModelsInflammatory DiseasesGene Expression StudiesGene Expression DataPhenotype FileMolecular Signature DatabaseDNA Chip AnnotationsGSEA SoftwareGene SymbolsPermutations

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