Journal
/
Biologie
/
Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

DOI:
10.3791/55809-v

•

07:28 min

•

June 19, 2017

•

, , , , ,
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Chapitres

  • 00:05Titre
  • 00:52Whitefly Rearing, Cryptic Species Identification, and Endosymbiont Identification and Localization
  • 02:11Whitefly Bacteriome Dissection and Purification
  • 03:47Amplification of Endosymbiont Genomes
  • 05:02Results: Extraction and Genome Sequencing of Endosymbionts from the Whitefly Bemisia tabaci
  • 06:12Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to isolate endosymbionts from the whitefly Bemisia tabaci through dissection and filtration. After amplification, the DNA samples are suitable for subsequent sequencing and study of the mutualism between endosymbionts and whitefly.

Tags

Keywords: EndosymbiontsWhitefly Bemisia TabaciPCRDNA ExtractionBacteriome DissectionInsect EndosymbiontsBacterial Isolation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/55809?article_type=v

Methods for Patch Clamp Capacitance Recordings from the Calyx
check_url/fr/55809?article_type=v

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region
check_url/fr/55809?article_type=v

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae
check_url/fr/55809?article_type=v

Methods for the Study of the Zebrafish Maxillary Barbel
check_url/fr/55809?article_type=v

Extraction of the EPP Component from the Surface EMG
check_url/fr/55809?article_type=v

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
check_url/fr/55809?article_type=v

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
check_url/fr/55809?article_type=v

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
check_url/fr/55809?article_type=v

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
check_url/fr/55809?article_type=v

Methods for Staging Pupal Periods and Measurement of Wing Pigmentation of Drosophila guttifera

Read Article