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Lysmediert reversibel modulering av den mitogenaktiverte proteinkinaseveien under celledifferensiering og<em> Xenopus</em> Embryonutvikling
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1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences,University of Illinois at Urbana-Champaign, 3Neuroscience Program,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign
Chapitres
- 00:05Titre
- 01:22Optogenetic Induction of Protein Localization in BHK21 Mammalian Cell Culture
- 04:10Construction of an LED Array for Long-term Light Stimulation in a CO2 Incubator
- 05:05Optogenetic Induction of PC12 Cell Differentiation
- 06:04Optogenetic Control of Kinase Activity in Xenopus Embryos
- 07:22Results: Optogenetic Induction of PC12 Cell Differntiation and Kinase Activity in Live Xenopus Embryos
- 09:00Conclusion
Denne protokollen beskriver en optogenetisk strategi for å modulere mitogenaktivert proteinkinase (MAPK) aktivitet under celledifferensiering og utvikling av Xenopus embryon. Denne metoden tillater reversibel aktivering av MAPK-signalveien i pattedyrcellekultur og i multicellulære levende organismer, som Xenopus- embryoer, med høy romlig og tidsmessig oppløsning.
