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Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
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Chapitres
- 00:05Titre
- 00:48Preparation of Late-stage Embryos for Immunostaining
- 04:19LacZ Staining and Immunostaining
- 07:20Preparing Embryos to Image the ISN Nerves in Abdominal Segments 7 to A9
- 10:03Results: Intersegmental Nerve Bundle (ISNb) Innervation at A3 and A4
- 11:21Conclusion
This work details a standard immunohistochemistry method to visualize motor neuron projections of late stage-16 Drosophila melanogaster embryos. The filleted preparation of fixed embryos stained with FasII antibody provides a powerful tool to characterize the genes required for motor axon pathfinding and target recognition during neural development.
