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Tredimensjonale vev utvikling justert astrocytter nettverk å Recapitulate utviklingsmessige mekanismer og tilrettelegge nervesystemet gjenfødelse
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1Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine,University of Pennsylvania, 2Center for Neurotrauma, Neurodegeneration & Restoration,Michael J. Crescenz Veterans Affairs Medical Center, 3School of Biomedical Engineering,Drexel University, 4Department of Bioengineering, School of Engineering and Applied Sciences,University of Pennsylvania, 5Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania
Chapitres
- 00:05Titre
- 01:03Agarose Hydrogel Micro-Column Fabrication
- 03:51ECM Core Fabrication
- 05:26Seeding Micro-columns with Astrocytes
- 06:32Results: Formation of Astrocyte Bundles within Hydrogel Micro-columns over Time
- 07:54Conclusion
Vi presentere utviklingen av selv montert, tredimensjonal bunter av langs justert astrocytic somata og prosesser i en roman biomateriale encasement. Disse konstruert "levende stillaser", viser mikron skala diameter men utvide centimetere i lengde, kan tjene som test senger å studere neurodevelopmental mekanismer eller lette neuroregeneration av dirigere neuronal overføringen og/eller axonal pathfinding.
Tags
Astrocyte NetworksAligned AstrocytesNervous System RegenerationMicrogel Micro-columnsGlial TubesRostral Migratory StreamAgarose HydrogelIn Vitro Neuro-biological ModelNeuro-regenerative TherapyCell MigrationAxon PathfindingGlial-mediated DevelopmentTraumaNeurodegenerative DiseaseCentral Nervous SystemRegenerationTreatment StrategiesNeuroanatomyGlia-mediated MechanismsAcupuncture NeedleCapillary TubeMicro-column BoatsAgarose SolutionDPBSMicro Scalpel
