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Den omvendte hjertemodel for interstitiell transudatsamling fra det isolerede rottehjerte
 
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Den omvendte hjertemodel for interstitiell transudatsamling fra det isolerede rottehjerte

Article DOI: 10.3791/55849-v • 07:59 min • June 20th, 2017
June 20th, 2017 •
*1,2, *1,2, 2, 3, 3, 1, 2
1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 2Department of Molecular Cardiology, Heinrich Heine University, 3Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University
* These authors contributed equally

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Denne protokol beskriver en metode til at indsamle hjerteinterstitielvæske fra det isolerede, perfuserede rottehjerte. For fysisk at adskille interstitialtransudat fra koronar venøs effluentperfusus, er Langendorff-perfusionshjertet omvendt, og transudatet (interstitialvæsken) dannet på hjerteoverfladen opsamles under anvendelse af en blød latexhætte.

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Medicin udgave 124 Langendorff perfusion omvendt hjerte rotte interstitiel væske myokardieinfarkt HPLC proteomics
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