Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:58Harvesting Spinal Cords
- 02:27Isolating Neurons
- 05:53Results: Immunofluorescent Staining of Neurons Using Neuronal Cytoskeletal Marker – MAP2
- 06:47Conclusion
This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic cleavage.
