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Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
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Journal JoVE Biologie
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

DOI:
10.3791/55870-v

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06:26 min

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June 19, 2017

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, , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery,University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty,University of Rostock

Chapitres

  • 00:05Titre
  • 00:38Transfection of Cardiomyocytes with Fluorescently Labeled miRNA
  • 01:56Applying 3D-FRAP Microscopy and Data Analysis
  • 04:08Results: 3D FRAP Analysis of Gap Junction-dependent Transfer of miRNA
  • 05:42Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

Tags

Gap JunctionMicro RNA3D-FRAP MicroscopyCardiomyocytesCell CultureElectroporationConfocal MicroscopyFluorescence Recovery After Photobleaching (FRAP)

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