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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
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Journal JoVE Bio-ingénierie
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

DOI:
10.3791/55873-v

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09:19 min

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September 15, 2017

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, , , ,
1Department of Biomedical Engineering,Rensselaer Polytechnic Institute, 2Center for Biotechnology and Interdisciplinary Studies,Rensselaer Polytechnic Institute, 3Department of Bioengineering,Northeastern University, 4Department of Bioengineering,University of Texas at Arlington

Chapitres

  • 00:05Titre
  • 00:52Preparation of Materials for Hydrogel Synthesis
  • 01:57Preparation of Sterile Glass Slide Molds for Hydrogel Formation
  • 03:04Hydrogel Formation
  • 04:06Protein Patterning
  • 05:18Cell Seeding onto Hydrogels
  • 06:58Results: Endothelial Cells Respond to VEGF Patterned Hydrogels
  • 08:31Conclusion

Summary

Traduction automatique

Traduction automatique

In this method, we use photopolymerization and click chemistry techniques to create protein or peptide patterns on the surface of polyethylene glycol (PEG) hydrogels, providing immobilized bioactive signals for the study of cellular responses in vitro.

Tags

Keywords: HydrogelBioactive ProteinsPeptidesPhotochemistrySpatial Protein PatterningRegenerative MedicineCell SignalingStem Cell FatePolyethylene Glycol DiacrylatePhotoinitatorFibronectinHydrogel MoldUV Sterilization

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