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ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
Journal JoVE
Biologie
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Journal JoVE Biologie
ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

DOI:
10.3791/55879-v

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11:19 min

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July 03, 2017

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, , ,
1Institute for Systems Biology, 2Center for Infectious Disease Research

Chapitres

  • 00:05Titre
  • 00:39Agar Mold Assembly
  • 03:36ODELAY Culture Preparation
  • 05:41Spotting on Agar Using Automated Liquid Spotting Robot
  • 07:21ODELAY Time-lapse Microscopy
  • 09:03Results: ODELAY Characterizes all 3 Growth Phase of Yeast Cells on Solid Media
  • 10:00Conclusion

Summary

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We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.

Tags

Keywords: ODELAYMulti-parameter QuantificationYeast GrowthHigh-throughputPopulation HeterogeneityAgar MoldColony Forming MicroorganismGenetic PerturbationsDrug InteractionsGrowth PhenotypesMicrobiologyGénétiqueSystems BiologyAcrylic MoldGlass SlideMolten AgarMold Separation

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