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诱导和诊断肿瘤<em>果蝇</em> Imaginal Disc Epithelia

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Recherche en cancérologie

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Journal JoVE Recherche en cancérologie

Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia

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诱导和诊断肿瘤<em>果蝇</em> Imaginal Disc Epithelia

DOI:

10.3791/55901-v

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08:14 min

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July 25, 2017

•

Kenta Morimoto², Yoichiro Tamori

¹Structural Biology Center, National Institute of Genetics and Department of Genetics, School of Life Science,SOKENDAI, ²Graduate School of Media and Governance,Keio University

Chapitres

00:05Titre
00:56Mosaic Clone Induction
02:21Dissection of Larvae
03:19Isolating and Mounting Imaginal Discs
04:29Diagnosing Neoplastic Phenotypes from Confocal Image
06:17Results: Tumors Induction from IgI-RNAi Expression in the Wing Disc
07:40Conclusion

Summary

Traduction automatique

果蝇成像盘上皮的马赛克克隆分析是研究肿瘤发生的遗传和细胞机制的有力模型系统。在这里，我们描述了使用GAL4-UAS系统在果蝇翼成像盘中诱导肿瘤的方案，并介绍了分类肿瘤表型的诊断方法。

Tags

Keywords: Tumor Induction Drosophila Imaginal Discs Mosaic Clones Tumor Development Heat-shock Flip-out GAL4 Third-instar Larvae Dissection Fixation Antibody Staining

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