Journal
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Sciences du comportement
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Automated Analysis of a Nematode Population-based Chemosensory Preference Assay
Journal JoVE
Sciences du comportement
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Journal JoVE Sciences du comportement
Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

Automated Analysis of a Nematode Population-based Chemosensory Preference Assay

DOI:
10.3791/55963-v

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09:44 min

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July 13, 2017

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, ,
1Division of Biology and Bioengineering,California Institute of Technology, 2Howard Hughes Medical Institute,California Institute of Technology

Chapitres

  • 00:05Titre
  • 00:53Behavior Chamber Assembly
  • 04:00Assay Preparation
  • 05:44Chemosensory Preference Assay
  • 08:06Results: Preference Index Measures Response of Nematodes to Various Treatments
  • 09:12Conclusion

Summary

Traduction automatique

Traduction automatique

We present a behavior recording setup and protocol that enables automated analysis of the nematode, Caenorhabditis elegans' preference for soluble compounds in a population-based assay. This article describes the construction of a behavior chamber, the behavioral assay protocol, and video analysis software usage.

Tags

NematodeChemosensory PreferenceC. ElegansBehavior RecordingVideo AnalysisAutomated AnalysisPopulation-based AssayNeurobiology ResearchGenetic MutantsBehavior ChamberCamera MountFabric CoverExtrusion BarsCorner Brackets

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