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Meget multiplexeret, superopløselig billeddannelse af T-celler ved hjælp af madSTORM
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Immunologie et infection
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Journal JoVE Immunologie et infection
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Meget multiplexeret, superopløselig billeddannelse af T-celler ved hjælp af madSTORM

DOI:
10.3791/55997-v

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08:43 min

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June 24, 2017

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, , , , ,
1Laboratory of Cellular & Molecular Biology, National Cancer Institute,National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute,National Institutes of Health

Chapitres

  • 00:05Titre
  • 00:36Preparation of Fluorescent Nano-diamond-coated Coverslips
  • 02:49Activation of Jurkat T Cells
  • 04:15Imaging Activated Jurkat T Cells Using madSTORM
  • 07:00Results: Multiplexed Super-resolution Image of Proteins in an Activated T Cell
  • 07:54Conclusion

Summary

Traduction automatique

Traduction automatique

Vi demonstrerer en metode til at vise flere molekyler inden for heterogene nanostrukturer ved nøjagtighed af enkeltmolekyler ved hjælp af sekventiel binding og eluering af fluorescensmærkede antistoffer.

Tags

Keywords: T CellsMadSTORMSuper-resolution ImagingMultiplexed ImagingSingle Molecule Localization MicroscopyAlexa 647Poly-L-lysineFluorescent NanodiamondsAnti-CD3 AntibodyJurkat T CellsHBS Solution

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