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Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
DOI:

11:29 min

July 10, 2017

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Chapitres

  • 00:05Titre
  • 00:46Genomic DNA Digestion
  • 01:39Genomic DNA Gel Electrophoresis
  • 02:56Gel Fluorescent Imaging and Hybridization
  • 05:17Hybridization with Telomere Probe
  • 07:36Gel Washing, Phospher Screen Exposure, and Radio Imaging
  • 09:08Results: Telomere Length is Quantified by a Modefied Terminal Restriction Fragment Analysis
  • 10:28Conclusion

Summary

Traduction automatique

In this article, a detailed protocol for quantifying telomere length using a modified terminal restriction fragment analysis is discussed that provides fast and efficient direct measurement of telomere length. This technique can be applied to a variety of cell sources of DNA for quantifying telomere length.

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