Journal
/
Biochimie
/
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

DOI:
10.3791/56013-v

•

10:04 min

•

July 06, 2017

•

, ,
1Department of Biochemistry and Molecular Biology,University of Texas Health Science Center at Houston

Chapitres

  • 00:05Titre
  • 01:04Nuclei Isolation from Cross-linked Skeletal Muscle
  • 04:11Sonication
  • 05:14ChIP
  • 07:53Results: Purification of High-quality Nuclei from Cross-linked Skeletal Muscle Suitable for Chromatin Immunoprecipitation
  • 08:51Conclusion

Summary

Traduction automatique

Traduction automatique

We present a filtration-based protocol to isolate high-quality nuclei from cross-linked mouse skeletal muscle wherein we removed the need for ultracentrifugation, making it easily applicable. We show that chromatin prepared from the nuclei is suitable for chromatin immunoprecipitation and likely chromatin immunoprecipitation sequencing studies.

Tags

Cross-linked Skeletal MuscleChromatin ImmunoprecipitationNuclei IsolationFiltration-based MethodMuscle Genomic StudiesTime-sensitive ContextFormaldehyde Cross-linkingDounce HomogenizerProtease Inhibitors

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56013?article_type=v

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
check_url/fr/56013?article_type=v

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
check_url/fr/56013?article_type=v

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
check_url/fr/56013?article_type=v

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
check_url/fr/56013?article_type=v

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
check_url/fr/56013?article_type=v

Preparation of High-Quality Fermented Fish Product
check_url/fr/56013?article_type=v

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
check_url/fr/56013?article_type=v

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
check_url/fr/56013?article_type=v

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
check_url/fr/56013?article_type=v

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Read Article