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Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
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Médecine
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Journal JoVE Médecine
Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

DOI:
10.3791/56018-v

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11:24 min

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February 10, 2018

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, , , , , , , , , ,
1National Institute of Immunology

Chapitres

  • 00:00Titre
  • 02:05Preparation
  • 03:40Procédure
  • 07:30Résultats
  • 09:56Precautions

Summary

Traduction automatique

Traduction automatique

We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdcscid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells.

Tags

Keywords: Hepatocyte TransplantationPartial HepatectomyNOD.SCID MiceCell EngraftmentCell-based TherapyGFP MouseNeoHep CellsIsoflurane AnesthesiaSurgical Procedure

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