Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male <em>C. Elegans </em>Head Neurons

Douglas K. Reilly; Daniel E. Lawler; Dirk R. Albrecht; Jagan Srinivasan

doi:10.3791/56026

/

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

DOI:

10.3791/56026-v

•

07:31 min

•

September 07, 2017

•

Douglas K. Reilly, Daniel E. Lawler, Dirk R. Albrecht², Jagan Srinivasan

¹Department of Biology and Biotechnology,Worcester Polytechnic Institute, ²Department of Biomedical Engineering,Worcester Polytechnic Institute

Chapitres

00:05Titre
00:41Microfluidic Device Set Up
02:54Loading C. elegans into the Microfluidic Device
04:44Olfactory Stimulation of C. elegans Using a Microfluidic Device
05:43Results: Neural Response to Chemical Stimuli
06:45Conclusion

Summary

Traduction automatique

The use of an adapted "olfactory chip" for the efficient calcium imaging of C. elegans males is described here. Studies of male exposure to glycerol and a pheromone are also shown.

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