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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
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Journal JoVE Biochimie
Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

DOI:
10.3791/56031-v

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06:36 min

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July 28, 2017

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, , , ,
1Department of Biomolecular Chemistry,University of Wisconsin School of Medicine and Public Health, 2Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School

Chapitres

  • 00:05Titre
  • 00:35Preparing the Horizontal Native Polyacrylamide Gel
  • 02:27Binding Reaction
  • 03:23Analysis of Samples
  • 04:12Imaging the Gel
  • 05:04Results: Horizontal Gel Electrophoresis Enhances the Detection of Distinct RNA-protein Complexes
  • 06:08Conclusion

Summary

Traduction automatique

Traduction automatique

Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.

Tags

Keywords: Horizontal Gel ElectrophoresisProtein-RNA ComplexesRNA BiochemistryRNA-protein InteractionsNative Gel ElectrophoresisGel BoxTBE Running BufferAcrylamide GelBinding ReactionFluorescently Labeled RNABicaudal-c Protein

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