Journal
/
Immunologie et infection
/
中和流感病毒单克隆抗体逃逸变种的产生
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Please note that all translations are automatically generated. Click here for the English version.

中和流感病毒单克隆抗体逃逸变种的产生

DOI:
10.3791/56067-v

•

07:55 min

•

August 29, 2017

•

, , , , , , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

Chapitres

  • 00:05Titre
  • 00:49Protocol 1: Generation of Escape Mutant Variants Against HI-positive/Neutralization-positive Antibodies
  • 02:51Protocol 2: Generation of Escape Mutant Variants Against HI-negative/Neutralization-positive Antibodies
  • 06:17Results: Epitope Map of the Novel Avian H7N9 HA Generated with Escape Mutant Variants
  • 07:11Conclusion

Summary

Traduction automatique

Traduction automatique

我们描述一种方法, 我们确定的关键残留物所需的结合的人或小鼠单克隆抗体的目标病毒血甲型流感病毒。该协议可以适应其他病毒表面糖蛋白及其相应的中和抗体。

Tags

Neutralizing AntibodiesMonoclonal AntibodiesEpitope MappingVirus Escape VariantsHemagglutination Inhibition (HI) AssayVirus NeutralizationEmbryonated Chicken EggsMDCK CellsVirus PassagingVirus-antibody Interactions

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

质粒DNA重组流感病毒的一代

富达的RNA病毒和病毒的突变频率的表征的变种的分离

流感病毒的高通量检测方法

在鸡胚的流感病毒传播

从排序的永生B细胞产生的重组人IgG单克隆抗体

鼠单克隆抗体的产生杂交瘤技术

用 MDCK-SIAT1 细胞 Microneutralization 法测定人血清中中和 (H3N2) 病毒的流感中和抗体反应

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

针对天然产物的单克隆抗体的产生

抗原脂质体用于生成特定于疾病的抗体

Read Article