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Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling
Journal JoVE
Médecine
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Journal JoVE Médecine
Screening Bioactive Nanoparticles in Phagocytic Immune Cells for Inhibitors of Toll-like Receptor Signaling
DOI:

09:51 min

July 26, 2017

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Chapitres

  • 00:05Titre
  • 00:57Preparation of Cell Culture Media and Reagents
  • 02:10Culture of THP-1 Reporter Cell-derived Macrophages
  • 03:45Screening for Potential TLR4 Nano-inhibitors Using the Reprter Cells
  • 07:22Results: Inhibition of Toll-like Receptor Signaling
  • 09:13Conclusion

Summary

Traduction automatique

Toll-like receptor (TLR) signaling plays an important role in the pathophysiology of many human inflammatory diseases, and regulating TLR responses by bioactive nanoparticles is anticipated to be beneficial in many inflammatory conditions. THP-1 cell-based reporter cells provide a versatile and robust screening platform for identifying novel inhibitors of TLR signaling.

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