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The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
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Journal JoVE Biologie du développement
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro

DOI:
10.3791/56083-v

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10:04 min

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September 18, 2017

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, , ,
1Create Fertility Centre, 2Department of Physiology,University of Toronto, 3Department of Obstetrics and Gynecology,University of Toronto, 4Department of Medical Sciences,University of Toronto, 5Department of Obstetrics and Gynecology,Women’s College Hospital

Chapitres

  • 00:05Titre
  • 01:07Aortic Ring Assay Setup
  • 02:30Embedding Aortic Rings in Basal Membrane Extract
  • 03:36Preparation of Aortic Ring and Mesenchymal Stromal Cells Co-culture assay
  • 04:49Flow Cytometry and qPCR
  • 07:09Results: Aortic Ring Assay with MSC
  • 08:48Conclusion

Summary

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Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.

Tags

Keywords: Aortic RingCo-culture AssayAngiogenic PotentialMesenchymal Stromal CellsRegenerative TherapyVascular TargetingIschemic DiseasesFunctional AssayIn VitroBasal Membrane Extract (BME)Aorta IsolationIntercostal ArteriesAortic RingsEmbedding

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