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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
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Journal JoVE Biologie
In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

DOI:
10.3791/56096-v

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09:40 min

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November 02, 2017

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, , ,
1Department of Ophthalmology,Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration,Fudan University

Chapitres

  • 00:05Titre
  • 00:54Cell Culture and Cell Cycle Synchronization
  • 03:18Immunoprecipitation of Endogenerous Rb Protein
  • 06:12Analysis of 6XHis-tagged Exogenous Rb SUMOylation in Human Cells
  • 08:20Results: Detection of Both Endogenous and Exogenous Rb SUMOylation
  • 09:08Conclusion

Summary

Traduction automatique

Traduction automatique

Small ubiquitin-related modifier (SUMO) family proteins are conjugated to the lysine residues of target proteins to regulate various cellular processes. This paper describes a protocol for the detection of retinoblastoma (Rb) protein SUMOylation under endogenous and exogenous conditions in human cells.

Tags

Rb Protein SUMOylationIn Vivo DetectionHuman CellsSUMO ConjugationCell SynchronizationG1 PhaseS PhaseG2/M PhaseHEK293 CellsCell Cycle AnalysisFlow CytometryRIPA Lysis

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