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The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
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Chapitres
- 00:05Titre
- 01:02Introduction to the Accompanying Publication
- 02:28RNAi Interaction Screen to Modify a Canal lumen Phenotype for Identification of Genes Involved in Intracellular Lumen Extension
- 03:29Scoring Excretory Canal Phenotypes using Fluorescence Dissecting Microscopy
- 05:20Imaging the C. elegans Excretory Canal using Confocal Microscopy
- 08:16Results: GFP Labeling, RNAi and Microscopic Analysis of Excretory Canal Lumen Morphogenesis Phenotypes
- 09:51Conclusion
The C. elegans excretory canal is a unique single-cell model for the visual in vivo analysis of de novo polarized membrane biogenesis. This protocol describes a combination of standard genetic/RNAi and imaging approaches, adaptable for the identification and characterization of molecules directing unicellular tubulogenesis, and apical membrane and lumen biogenesis.
