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Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling
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Biologie
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Journal JoVE Biologie
Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

DOI:
10.3791/56118-v

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13:32 min

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March 04, 2018

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, , , , ,
1Department of Aquaculture,Szent István University

Chapitres

  • 00:00Titre
  • 01:14Testis collection
  • 03:29Ultra-rapid cooling of the testicular tissue
  • 06:11Warming procedure
  • 07:56Tissue digestion
  • 10:27Viability evaluation and cell counting
  • 11:28Representative Results
  • 12:20Conclusions

Summary

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Traduction automatique

The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Additionally, the repeatability of the method in five different zebrafish strains was tested.

Tags

CryopreservationZebrafishSpermatogoniaVitrificationWhole TestesUltra-rapid CoolingGerm CellsTransplantationSlow-rate FreezingSpermatozoaEuthanasiaDissectionTestes RemovalSterilizationEquilibrationVitrification Solutions

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