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Biologie du développement
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Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
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Biologie du développement
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Journal JoVE Biologie du développement
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae

DOI:
10.3791/56121-v

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12:30 min

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October 06, 2017

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, , , ,
1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health,Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children’s Hospital

Chapitres

  • 00:05Titre
  • 01:27Designing an Artificial Intronic Small RNA Expression Cassette
  • 04:22Generating a rAaeDV Construct by Cloning a miRNA or shRNA Expression Cassette into a Plasmid Backbone
  • 05:19Transfecting C6/36 Cells with rAaeDV Plasmids
  • 07:33Harvesting rAaeDV Virions from Transfected C6/36 Cells
  • 08:53Mosquito Transduction
  • 10:10Results: Overexpression and Knockdown Efficiency of rAaeDV Vectors
  • 11:14Conclusion

Summary

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Traduction automatique

We report using an artificial intronic small RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus (AaeDV) in vivo delivery system. A detailed procedure for the construction, packaging, and quantitative analysis of the rAaeDV vectors as well as for larval infection is described.

Tags

Keywords: Recombinant Mosquito DensovirusGene Delivery VectorFunctional AnalysisMosquito LarvaeViral-based DeliveryTransduction EfficiencyPersistent EffectsMiRNAShRNAAedes AegyptiGene FunctionGene OverexpressionGene Knockdown

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