Journal
/
Immunologie et infection
/
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

DOI:
10.3791/56130-v

•

08:37 min

•

September 22, 2017

•

, ,
1Department of Veterinary and Biomedical Sciences,University of Minnesota

Chapitres

  • 00:05Titre
  • 00:57Fresh Tissue Sectioning
  • 02:25Primary, Secondary, and Tertiary Antibody (Ab) Incubation
  • 04:37Sample Mounting and Confocal Microscopic Imaging
  • 05:47Results: Representative MHC-tetramer Staining
  • 07:25Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.

Tags

Keywords: In SituMHC-tetramer StainingAntigen-specific CD8 T CellsQuantitative AnalysisImmunofluorescence StainingMicroscopy

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/56130?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/fr/56130?article_type=v

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
check_url/fr/56130?article_type=v

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
check_url/fr/56130?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/fr/56130?article_type=v

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
check_url/fr/56130?article_type=v

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
check_url/fr/56130?article_type=v

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
check_url/fr/56130?article_type=v

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
check_url/fr/56130?article_type=v

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
check_url/fr/56130?article_type=v

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Read Article