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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Journal JoVE
Biologie
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Journal JoVE Biologie
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
DOI:

10:17 min

October 05, 2017

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Chapitres

  • 00:05Titre
  • 01:01Macroinvertebrate Sample Preparation for Genetic Gut Content Analysis
  • 03:56Verification of Functional Efficiency of DNA Extracts
  • 05:37Automated Fragment Analysis for Parallel Detection of Multiple, Recently Ingested Prey Groups
  • 08:12Results: Predator Diet Analyses Using Group-specific Prey DNA Primers
  • 09:40Conclusion

Summary

Traduction automatique

Most common gut content analyses of macroinvertebrates are visual. Requiring intense knowledge about morphological diversity of prey organisms, they miss soft bodied prey and, due to strong comminution of prey, are nearly impossible for some organisms, including amphipods. We provide detailed, novel genetic approaches for macroinvertebrate prey identification in the diet of amphipods.

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