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Laboratoriet protokoll for genetisk Gut innhold analyser av akvatiske Macroinvertebrates bruke gruppe-spesifikke rDNA primere
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Journal JoVE Biologie
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Laboratoriet protokoll for genetisk Gut innhold analyser av akvatiske Macroinvertebrates bruke gruppe-spesifikke rDNA primere

DOI:
10.3791/56132-v

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10:17 min

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October 05, 2017

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1Institute for Environmental Sciences,University of Koblenz-Landau, 2Institute of Integrated Natural Sciences,University of Koblenz-Landau, 3IV 2.5 (Trace Analysis, Artificial Pond and stream system),Federal Environment Agency

Chapitres

  • 00:05Titre
  • 01:01Macroinvertebrate Sample Preparation for Genetic Gut Content Analysis
  • 03:56Verification of Functional Efficiency of DNA Extracts
  • 05:37Automated Fragment Analysis for Parallel Detection of Multiple, Recently Ingested Prey Groups
  • 08:12Results: Predator Diet Analyses Using Group-specific Prey DNA Primers
  • 09:40Conclusion

Summary

Traduction automatique

Traduction automatique

Vanligste gut innhold analyser av macroinvertebrates er visuelle. Krever intens kunnskap om morfologiske mangfold av byttedyr organismer, de savner myk bodied byttedyr og på grunn av sterk comminution av byttedyr, er nesten umulig for noen organismer, inkludert amfipoder. Vi gir detaljert, romanen genetiske metoder for macroinvertebrate byttedyr identifikasjon i kostholdet av amfipoder.

Tags

Keywords: Genetic Gut Content AnalysisAquatic MacroinvertebratesRDNA PrimersTrophic EcologyFood WebInvasive SpeciesDNA ExtractionProteinase KSDSSodium ChlorideIsopropanolPCRPrimer Design

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