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The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury
 

The Murine Choline-Deficient, Ethionine-Supplemented (CDE) Diet Model of Chronic Liver Injury

Article DOI: 10.3791/56138-v • 07:28 min • October 21st, 2017
October 21st, 2017 •
1, 1, 2, 3, 1,4, 1, 5,6,7, 8,9, 10,11, 1,12
1School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, 2School of Public Health & Curtin Health Innovation Research Institute, Curtin University, 3Institute of Biochemistry, Christian-Albrechts-University, 4School of Veterinary and Life Sciences, Murdoch University, 5Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, 6Royal Prince Alfred Hospital, 7A.W. Morrow Gastroenterology and Liver Centre, 8QIMR Berghofer Medical Research Institute, 9Faculty of Medicine and Biomedical Sciences, The University of Queensland, 10Fiona Stanley and Fremantle Hospitals, 11School of Medical and Health Sciences, Edith Cowan University, 12School of Medicine and Pharmacology, University of Western Australia

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Here we describe a common method to induce chronic liver injury in mice by feeding of a choline-deficient and ethionine-supplemented (CDE) diet. We demonstrate health monitoring, liver perfusion, isolation, and preservation. A time course of six weeks can inform about liver injury, pathohistology, fibrosis, inflammatory, and liver progenitor cell responses.

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Keywords: Choline-deficient Diet Ethionine-supplemented Diet Chronic Liver Injury Murine Model Steatosis Fatty Liver Inflammation Liver Progenitor Cell Proliferation Hepatic Fibrosis Liver Regeneration Chronic Liver Disease Cardio Puncture Liver Perfusion Body Weight Greasy Coat Anesthesia Cardiac Puncture
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