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Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary
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Biologie
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Journal JoVE Biologie
Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

Three-dimensional Reconstruction of the Vascular Architecture of the Passive CLARITY-cleared Mouse Ovary

DOI:
10.3791/56141-v

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12:38 min

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December 10, 2017

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, , ,
1Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences; Institutes of Brain Science, Brain Science Collaborative Innovation Center, State Key Laboratory of Medical Neurobiology, Institute of Acupuncture and Moxibustion, Fudan Institutes of Integrative Medicine,Fudan University, 2Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine,Stanford University

Chapitres

  • 00:00Titre
  • 01:36Perfusion and Ovary Preparation
  • 03:00Degassing and Passive CLARITY
  • 05:01Immunostaining
  • 05:50Confocal Imaging
  • 08:373D Reconstruction of Ovarian Structure
  • 11:04Results: Representative 3D Passive CLARITY Images of Cleared Ovary
  • 12:00Conclusion

Summary

Traduction automatique

Traduction automatique

Here we present an adaptation of the passive CLARITY and 3D reconstruction method for visualization of the ovarian vasculature and follicular capillaries in intact mouse ovaries.

Tags

Keywords: 3D ReconstructionVascular ArchitectureCLARITY-clearedMouse OvaryFollicle DevelopmentSomatic CellsVascular SystemNervous SystemPassive CLARITYImage AnalysisImarisPerfusionHydrogelOvarian Bursa

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