Journal
/
Recherche en cancérologie
/
Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice
Journal JoVE
Recherche en cancérologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Recherche en cancérologie
Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

DOI:
10.3791/56159-v

•

08:57 min

•

October 05, 2017

•

, , , , , , ,
1Department of Pharmaceutical Sciences,University of Toronto, 2Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute,University Health Network

Chapitres

  • 00:05Titre
  • 01:20Biological Sample Preparation
  • 03:35HPLC Instrumentation and Operation Parameters
  • 04:50Analysis of Samples
  • 05:46Results: Drug Combination Delivery of Doxorubicin and Mitomycin C in Nanoparticles
  • 07:43Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes an efficient and convenient analytical process of sample extraction and simultaneous determination of multiple drugs, doxorubicin (DOX), mitomycin C (MMC) and a cardio-toxic DOX metabolite, doxorubicinol (DOXol), in the biological samples from a preclinical breast tumor model treated with nanoparticle formulations of synergistic drug combination.

Tags

DoxorubicinMitomycin CDoxorubicinolNanoparticlesTumorHPLCSimultaneous QuantitationSample PreparationExtractionInternal Standard

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

The Colon-26 Carcinoma Tumor-bearing Mouse as a Model for the Study of Cancer Cachexia

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

Evaluation of the In vivo Antitumor Activity of Polyanhydride IL-1α Nanoparticles

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Read Article