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Flow flowcytometri-baserede stof Screening System til identifikation af små molekyler, der fremmer Celledifferentiering glioblastom stamceller
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1Department of Neurological Surgery, Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine, 2Department of Pathology,Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine
Chapitres
- 00:05Titre
- 01:17Generation of Lentiviral Transcriptional Reporter Cell Lines
- 03:47Subclone Selection, Expansion, and Validation
- 04:47Determination of GFAP:GFP Expression by Flow Cytometry
- 05:58ELDA Self-renewal Assay to Assess Clonogenic Capacity
- 07:01Drug Screen
- 08:27Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
- 09:46Conclusion
En effektiv screening protokol præsenteres til identifikation af små molekyler, der fremmer astroglial differentiering i glioblastom stamceller (GSCs). Analysen er baseret på en stamcelle differentiering reporter hvorved udtryk for forbedrede normal god landbrugspraksis (eGFP) er drevet af den menneskelige GFAP promotor.
Tags
Flow CytometryDrug ScreeningGlioblastoma Stem CellsCellular DifferentiationLentivirusReporter ConstructHigh-throughput ScreeningSmall MoleculesAstroglial DifferentiationCancer Stem CellsEhlers-Danlos GlioblastomaCell LineageBloodborne PathogensNeural Stem Cell Growth MediumPolybreneDissociation Reagent
