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Flow cytometri-baserte narkotika Screening System for identifisering av små molekyler som fremmer differensiering av glioblastom stilk celler
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1Department of Neurological Surgery, Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine, 2Department of Pathology,Johns Hopkins University, School of Medicine, 3Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center,Case Western Reserve University School of Medicine
Chapitres
- 00:05Titre
- 01:17Generation of Lentiviral Transcriptional Reporter Cell Lines
- 03:47Subclone Selection, Expansion, and Validation
- 04:47Determination of GFAP:GFP Expression by Flow Cytometry
- 05:58ELDA Self-renewal Assay to Assess Clonogenic Capacity
- 07:01Drug Screen
- 08:27Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones
- 09:46Conclusion
En effektiv screening protokoll er presentert for identifikasjon av små molekyler som fremmer astroglial differensiering i glioblastom stilk celler (GSCs). Analysen er basert på en stilk cellen differensiering reporter der uttrykket av den forbedrede GFP (eGFP) er drevet av menneskelig GFAP selskapet.
Tags
Flow CytometryDrug ScreeningGlioblastoma Stem CellsCellular DifferentiationLentivirusReporter ConstructHigh-throughput ScreeningSmall MoleculesAstroglial DifferentiationCancer Stem CellsEhlers-Danlos GlioblastomaCell LineageBloodborne PathogensNeural Stem Cell Growth MediumPolybreneDissociation Reagent
