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Chapitres
- 00:05Titre
- 00:57Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
- 04:12Crosslinking Proteins to DNA
- 05:01Lysis, Sonication, and Immunoprecipitation
- 06:33Washing, Elution, and Reversal of Crosslinking
- 08:57Cellular Protein and RNA Digestion
- 10:06Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
- 11:48Conclusion
Tags
Keywords:
Chromatin Immunoprecipitation (ChIP)Low-abundance Embryonic SamplesHistone ModificationsVertebrate GenomesBiologie du développementEnhancersGenesTranscription RegulationCellular HeterogeneityPatient SamplesChicken EmbryosHH19Extra-embryonic MembranesSpinal Neuro Tube (SNT)TrypsinizationDMEMFBS
