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1Department of Biological Chemistry, School of Medicine,University of California, Irvine, 2Beckman Laser Institute and Medical Clinic,University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences,University of California, Irvine, 4Department of Biomedical Engineering and Surgery,University of California, Irvine
Chapitres
- 00:05Titre
- 01:25Transient Transfection
- 04:09Titration of Laser Input Power
- 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
- 06:54Cell Cycle and G1 Phase Synchronization
- 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
- 09:27Conclusion
Målet med denne protokol er at beskrive, hvordan du bruger laser microirradiation til at fremkalde forskellige typer af DNA-skader, herunder relativt simple strand pauser og komplekse skader, at studere DNA skader signalering og reparation faktor forsamling på skade steder in vivo .
