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斑马鱼幼虫动物模型的建立研究锥虫运动性体内
Journal JoVE
Biologie du développement
Author Produced
This content is Free Access.
Journal JoVE Biologie du développement
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
DOI:

13:21 min

September 30, 2017

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Chapitres

  • 00:00Titre
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Traduction automatique

在本协议中, 荧光标记为T. 虫被注射到透明的斑马鱼幼虫中, 并使用光片荧光显微镜观察到体内的寄生虫运动。

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