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Ground State Depletion Super-resolution Imaging in Mammalian Cells
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Journal JoVE Biologie
Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

DOI:
10.3791/56239-v

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07:55 min

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November 05, 2017

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, , ,
1Department of Physiology and Membrane Biology,University of California School of Medicine, Davis

Chapitres

  • 00:05Titre
  • 01:00GSD Super-resolution Imaging Photoswiching Buffer Preparation
  • 02:22Mounting of the Samples
  • 02:57Image Acquisition
  • 04:38Image Analysis
  • 06:07Results: Super-resolution GSD Imaging Improves Resolution
  • 07:08Conclusion

Summary

Traduction automatique

Traduction automatique

This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.

Tags

Super-resolution MicroscopyGround State DepletionGSDIMImmunocytochemistryProtein DistributionMembrane BiophysicsAlexa 647Fluorescence ImagingOxygen ScavengingPhotoswitchingThiol Solution

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