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Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease
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Médecine
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Journal JoVE Médecine
Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease

DOI:
10.3791/56290-v

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08:38 min

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August 26, 2017

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, , , , , , , ,
1Department of Cardiology and Angiology,University of Magdeburg, 2Leibniz Institute for Neurobiology, 3Institute of Molecular and Clinical Immunology,University of Magdeburg, 4Division of Hematology/Oncology,Boston Children’s Hospital, Harvard Medical School

Chapitres

  • 00:00Titre
  • 00:39Cell Preparation
  • 01:54Anesthesia
  • 02:00Implantation of Basement Membrane-like Matrix
  • 03:35Tail Vein Injection
  • 05:04Intravital Microscopy
  • 06:07Representative Results
  • 07:27Conclusion

Summary

Traduction automatique

Traduction automatique

Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model.

Tags

Keywords: Intravital MicroscopyMonocyte HomingTumor-related AngiogenesisPeripheral Arterial DiseaseCell LabelingMatrigelVEGFBFGFHeparinSubcutaneous InjectionSystemic Cell Application

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