Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Rosa G&#243;mez-Villafuertes; Luc&#237;a Paniagua-Herranz; Sergio Gascon; David de Agust&#237;n-Dur&#225;n; Mar&#237;a de la O Ferreras; Juan Carlos Gil-Redondo; Mar&#237;a Jos&#233; Queipo; Aida Menendez-Mendez; R&#225;quel P&#233;rez-Sen; Esmerilda G. Delicado; Javier Gualix; Marcos R. Costa; Timm Schroeder; Mar&#237;a Teresa Miras-Portugal; Felipe Ortega

doi:10.3791/56291

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Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Journal JoVE
Neurosciences

This content is Free Access.

Journal JoVE Neurosciences

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

DOI:

10.3791/56291-v

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10:55 min

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December 16, 2017

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Rosa Gómez-Villafuertes*^1,2,3, Lucía Paniagua-Herranz*^1,2,3, Sergio Gascon*^4,5, David de Agustín-Durán^2,3, María de la O Ferreras^2,3, Juan Carlos Gil-Redondo^2,3, María José Queipo^2,3, Aida Menendez-Mendez^2,3, Ráquel Pérez-Sen^2,3, Esmerilda G. Delicado^2,3, Javier Gualix^2,3, Marcos R. Costa, Timm Schroeder, María Teresa Miras-Portugal^2,3, Felipe Ortega^2,3

¹Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine,Complutense University, ²University Institute for Neurochemistry Research (IUIN), ³Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), ⁴Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center,Ludwig-Maximilians University Munich, ⁵Toxicology and Pharmacology Department, Faculty of Veterinary medicine,Complutense University, ⁶Brain Institute,Federal University of Rio Grande do Norte, ⁷Department of Biosystems Science and Engineering,Eidgenössische Technische Hochschule (ETH) Zurich

Chapitres

00:05Titre
01:14Live Imaging by Time-lapse Video-microscopy
04:02Post-imaging Immunocytochemistry, Data Collection, and Processing
06:38Single-cell Tracking
08:56Results: Example of aNSCs Isolated from the SEZ and Analyzed by Live Imaging and Single Cell Tracking
10:17Conclusion

Summary

Traduction automatique

A robust protocol to monitor neural populations by time-lapse video-microscopy followed by software-based post-processing is described. This method represents a powerful tool to identify biological events in a selected population during live imaging experiments.

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

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Chapitres

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