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In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
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Journal JoVE Médecine
In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

DOI:
10.3791/56301-v

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08:40 min

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November 22, 2017

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, , , , , , , , , , , , ,
1Laboratory of Molecular Gerontology, National Institute on Aging,National Institutes of Health, 2Institute of Molecular Biology and Biotechnology,Foundation for Research and Technology – Hellas, 3Center for Molecular Medicine, National Heart Lung and Blood Institute,National Institutes of Health, 4Laboratory of Neurosciences, National Institute on Aging,National Institutes of Health, 5Nuffield Department of Obstetrics and Gynaecology,University of Oxford, 6Department of Clinical Molecular Biology,University of Oslo and Akershus University Hospital, 7Department of Basic Sciences, Faculty of Medicine,University of Crete, 8Danish Center for Healthy Aging,University of Copenhagen

Chapitres

  • 00:05Titre
  • 01:30Detection of Mitophagy in Human Cells using mt-Keima Plasmid
  • 03:12Assessing Mitophagy using Co-localization Between DCT-1 and LGG-1 in C. elegans
  • 05:36Detection of Mitophagy in Mice
  • 06:19Results: Evaluation of Mitophagy in Human Cells, C. elegans, and Mice
  • 07:57Conclusion

Summary

Traduction automatique

Traduction automatique

Mitophagy, the process of clearing damaged mitochondria, is necessary for mitochondrial homeostasis and health maintenance. This article presents some of the latest mitophagy detection methods in human cells, Caenorhabditis elegans, and mice.

Tags

MitophagyIn VitroIn VivoHuman CellsC. ElegansMiceNeuro-degenerationMetabolic DiseasesAgingMito-KeimaConfocal MicroscopyTransfectionCell Culture

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