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用集成电极直接量化 Transendothelial 电阻的 Organ-on-chip 系统的制作与验证
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
DOI:

10:51 min

September 26, 2017

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Chapitres

  • 00:05Titre
  • 01:11Fabrication of the Organ-on-chip Device
  • 05:37On-chip Culture of Brain-derived Endothelial Cells
  • 08:42Results: Representative Impedence Data, TEER Determination and Development of BBB-on-chip
  • 10:04Conclusion

Summary

Traduction automatique

这本出版物描述了一个 organ-on-chip 装置的制造与集成电极直接量化的 transendothelial 电阻 (TEER)。为验证, 在该微流控装置内模拟血脑屏障, 监测其屏障功能。所提出的电极集成方法和直接 TEER 定量是普遍适用的。

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