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Basato su Dendrimero Nanopatterns irregolare alla localmente adesività di superficie di controllo: un metodo per dirigere la differenziazione Chondrogenic
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1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 2Department of Engineering Electronics,University of Barcelona (UB), 3Networking Biomedical Research Center (CIBER), 4Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry,Universidad de Málaga (UMA), 5Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, 6Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas,Universidade da Coruña (UDC), 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 8Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology,Universidad de Málaga (UMA)
Chapitres
- 00:00Titre
- 01:12Production of Nanopatterned Growth Substrates
- 05:59Local RGD Surface Density Characterization with AFM
- 07:49Cell Culture and Induction of Chondrogenesis
- 08:49Cell Fixation and Immunostaining
- 10:18Cell Imaging and Data Analysis
- 12:28Representative Results
- 13:29Conclusion
Un metodo per ottenere nanopatterns irregolare Dendrimero-based che consentono il controllo su scala nanometrica di locale arginina-glicina-aspartico (RGD) superficie densità dell'acido è descritto e applicato per lo studio del differenziamento cellulare adesione e chondrogenic.
