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Immunologie et infection
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Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
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Immunologie et infection
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Journal JoVE Immunologie et infection
Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos

DOI:
10.3791/56352-v

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10:06 min

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August 03, 2017

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, , ,
1Graduate School of Medical Sciences,Kanazawa University

Chapitres

  • 00:05Titre
  • 00:58Stage 16 Embryo Collection
  • 02:39Embryonic Preparation
  • 04:50Hemocyte and TUNEL Staining
  • 07:41Results: Representative Hemocyte and Apoptotic Cell Staining
  • 09:03Conclusion

Summary

Traduction automatique

Traduction automatique

We herein describe a phagocytosis assay using the dispersed embryonic cells of Drosophila. It enables us to easily and precisely quantify in vivo phagocytosis levels, and to identify new molecules required for the phagocytosis of apoptotic cells.

Tags

Keywords: PhagocytosisApoptotic CellsDrosophila EmbryosImmune ResponseBiologie du développementCell EngulfmentInflammatory DisordersAutoimmune DiseaseCell IsolationCollagenaseCell SuspensionCell HomogenizationCell Trituration

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