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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
DOI:

09:57 min

March 05, 2018

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Chapitres

  • 00:05Titre
  • 01:06Bone Marrow Aspiration and Smear Preparation
  • 03:16Flow Cytometry Setup
  • 05:53Cell Staining for Flow Cytometry
  • 07:45Results: Representative Leukemia-associated Immunophenotype (LAIP) Gating
  • 08:46Conclusion

Summary

Traduction automatique

Detection of minimal or measurable residual disease (MRD) is an important prognostic biomarker for refining risk assessment and predicting relapse in acute myeloid leukemia (AML). These comprehensive guidelines and recommendations with best practices for consistent and accurate identification and detection of MRD, may aid in making effective AML treatment decisions.

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