Journal
/
/
At studere hypothalamus Insulin Signal til perifere glukose Intolerance med en løbende Drug Infusion System ind i musen hjernen
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
DOI:

08:32 min

January 04, 2018

,

Chapitres

  • 00:05Titre
  • 01:21Preparation of Micro-osmotic Pump Infusion Systems
  • 02:41Intracerebral Ventricular Surgery – Implantation of the Micro-osmotic Pump
  • 04:15Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
  • 06:04Results: Glucose Metabolism and Insulin Signal Activation
  • 07:27Conclusion

Summary

Traduction automatique

Denne protokol undersøgelser af chemokine (C-C motiv) ligand 5 (CCL5) i hypothalamus rolle ved at levere en antagonist, MetCCL5, ind i musen hjernen ved hjælp af en mikro-osmotisk pumpe hjernen infusion system. Denne forbigående hæmning af CCL5 aktivitet afbrudt hypothalamus insulin signalering, fører til glukose intolerance og perifere systemisk insulinfølsomhed.

Vidéos Connexes

Read Article