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Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
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Journal JoVE Biologie du développement
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications

DOI:
10.3791/56419-v

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08:41 min

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December 04, 2017

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, , , , , ,
1Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

Chapitres

  • 00:05Titre
  • 00:43Sectioning Human Myocardium into Slices
  • 02:27Decellularization of Human Myocardium Slices
  • 04:20Processing the Decellularized cECM to a Powder
  • 05:50Enzyme-based Homogenization of cECM Micro-particles
  • 06:56Results: Effective Removal of Cellular Material Enhances Analysis
  • 08:10Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes the complete decellularization of human myocardium while preserving its extracellular matrix components. Further processing of the extracellular matrix results in the production of microparticles and a cytoprotective self-assembling hydrogel.

Tags

Keywords: Cardiac Extracellular MatrixHydrogelCell-based AssaysMyocardial InfarctionCryosectioningDecellularizationSDS

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