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Biologie du développement
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Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
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Biologie du développement
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Journal JoVE Biologie du développement
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

DOI:
10.3791/56441-v

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09:17 min

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April 12, 2018

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, , ,
1Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center,University of Zurich

Chapitres

  • 00:04Titre
  • 00:56Flower and Silique Fixation and Dissection Under the Stereomicroscope
  • 02:05Chloral Hydrate-based Clearing and Combined Clearing-staining
  • 03:28Combined Alexander Staining and Herr’s 4½ Clearing of Anthers
  • 04:29Removing the Exine from Pollen Grains
  • 05:49Sodium Dodecyl Sulfate (SDS) Clearing
  • 07:04Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures
  • 08:41Conclusion

Summary

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Traduction automatique

In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive structures.

Tags

Keywords: ArabidopsisFlower OrgansSiliquesWhole-mount ClearingStainingDissectionFlower DevelopmentSexual Plant ReproductionMicroscopyClearing Solution

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