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Evaluating In Vitro DNA Damage Using Comet Assay
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Journal JoVE Recherche en cancérologie
Evaluating In Vitro DNA Damage Using Comet Assay

Evaluating In Vitro DNA Damage Using Comet Assay

DOI:
10.3791/56450-v

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10:43 min

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October 11, 2017

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, ,
1Neuro-Oncology Branch, Center for Cancer Research,National Cancer Institute, 2Basic Medical Science Department,Zunyi Medical College-Zhuhai Campus

Chapitres

  • 00:05Titre
  • 00:46Preparing Comet Slides
  • 03:17Single Cell Electrophoresis
  • 05:43Staining Comet Slides
  • 06:25Image Acquisition and Analysis
  • 08:35Results: Combining Olaparib with Temozolomide Enhances DNA damage in Glioma Cells
  • 09:41Conclusion

Summary

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Traduction automatique

The comet assay is an efficient method to detect DNA damage including single and double-stranded DNA breaks. We describe alkaline and neutral comet assays to measure DNA damage in cancer cells to evaluate the therapeutic effect of chemotherapy.

Tags

Comet AssayDNA DamageGenotoxic AgentsCancer CellsSingle Cell ElectrophoresisAlkaline Comet AssayAgaroseCell CultureGlioma CellsChemotherapyLysis SolutionDNA UnwindingElectrophoresis

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