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Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
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Journal JoVE Biologie
Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

DOI:
10.3791/56475-v

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07:53 min

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January 16, 2018

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, ,
1Department of Biology,Temple University

Chapitres

  • 00:05Titre
  • 00:32NIH-3T3 Cell Preparation
  • 03:30SPEED Microscopy
  • 05:48Results: 3D Spatial Location of the Transport Route for SSTR3 in Ciliary Transition Zone and IFT20 Inside the Cilia Shaft
  • 07:18Conclusion

Summary

Traduction automatique

Traduction automatique

Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.

Tags

High-speed Super-resolution SPEED MicroscopyPrimary CiliaProtein TransportLive CellsNIH-3T3 CellsCell CultureTransfectionSSTR3 ConstructAlexa 647 Conjugated StreptavidinLive-cell Imaging

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