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Chapitres
- 00:05Titre
- 00:57Sample Procurement and Processing
- 02:29Immunohistochemistry
- 05:02Hematoxylin and Eosin Staining
- 06:19Ductal Analysis
- 08:33Immunohistochemictry Analysis
- 09:43Results: Whole Mouse Mammary Gland Isolation and Analysis
- 11:23Conclusion
Here, we present a protocol for the isolation of whole, intact mouse mammary glands to investigate extracellular matrix (ECM) expression and ductal morphology. Mouse #4 abdominal glands were extracted from 8-10 week old female nulliparous mice, fixed in neutral buffered formalin, sectioned and stained using immunohistochemistry for ECM proteins.
